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NASC

The European Arabidopsis Stock Centre

Agrikola pAgrikola Delivery Clones

Donated by

  • Thomas Altmann Agrikola consortium, Developmental Physiology and Genomics Group, Cooperative Research Groups, Max-Planck-Institut für Molekulare Pflanzenphysiologie

Description

Agrikola pAgrikola Delivery Clones

For further information please refer to the AGRIKOLA main page

pDelivery clone stabs

The Agrikola RNAi delivery clones are distributed as an LBA stab.

The following information was taken from the Agrikola website :

The AGRIKOLA consortium has cloned most of the CATMA GSTs into pAGRIKOLA, a Gateway® destination vector based closely on the Hellsgate 12 vector developed by Chris Helliwell, Peter Waterhouse and colleagues. These expression clones are in E. coli DH5a. pAGRIKOLA confers resistance to kanamycin in bacteria.

Before going any further you should streak the bacteria out on agar plates containing 50 mg.l-1 kanamycin and select 4 or 6 individual colonies for verification.
Knowing the size of your chosen GST, you can verify the plasmid by restriction digest using the pAGRIKOLA restriction map or better still by PCR amplification and/or sequencing.