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NASC

The European Arabidopsis Stock Centre

Berner, Heijmans, Agenent

Donated by

  • Gerco Angenent Business Unit Bioscience, Plant Research International B.V., Wageningen University
  • Klass Heijmans Laboratory of Plant Genetics, Experimental Botany Group, Institute for Wetland and Water Research (IWWR), Faculty of Science, Radboud University Nijmegen
  • Marian Berner Laboratory of Plant Cell Biology, Experimental Botany Group, Institute for Wetland and Water Research (IWWR), Faculty of Science, Radboud University Nijmegen

Click here to view all 43 of these lines.

Description

This is a collection of lines donated by Marian Berner, Gerco Angenent and Klass Heijmans. These lines are available to order individually or as a set

Nasc code Description Stock contents
N799467 Complete set of 43 lines View set contents

Abstract from Berner et al. (2010) relating to these lines:

An atlas of type I MADS box gene expression during female gametophyte and seed development in Arabidopsis.

Bemer M, Heijmans K, Airoldi C, Davies B, Angenent GC.

Plant Physiol. 2010 Sep;154(1):287-300. Epub 2010 Jul 14.

Members of the plant type I MADS domain subfamily have been reported to be involved in reproductive development in Arabidopsis (Arabidopsis thaliana). However, from the 61 type I genes in the Arabidopsis genome, only PHERES1, AGAMOUS-LIKE80 (AGL80), DIANA, AGL62, and AGL23 have been functionally characterized, which revealed important roles for these genes during female gametophyte and early seed development. The functions of the other genes are still unknown, despite the fact that the available single T-DNA insertion mutants have been largely investigated. The lack of mutant phenotypes is likely due to a considerable number of recent intrachromosomal duplications in the type I subfamily, resulting in nonfunctional genes in addition to a high level of redundancy. To enable a breakthrough in type I MADS box gene characterization, a framework needs to be established that allows the prediction of the functionality and redundancy of the type I genes. Here, we present a complete atlas of their expression patterns during female gametophyte and seed development in Arabidopsis, deduced from reporter lines containing translational fusions of the genes to green fluorescent protein and beta-glucuronidase. All the expressed genes were revealed to be active in the female gametophyte or developing seed, indicating that the entire type I subfamily is involved in reproductive development in Arabidopsis. Interestingly, expression was predominantly observed in the central cell, antipodal cells, and chalazal endosperm. The combination of our expression results with phylogenetic and protein interaction data allows a better identification of putative redundantly acting genes and provides a useful tool for the functional characterization of the type I MADS box genes in Arabidopsis.

References

  • Berner, M. et al. 2010. An atlas of type I MADS box gene expression during female gametophyte and seed development in Arabidopsis. Plant Physiology 154(1):287-300. PMID. 20631316.