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NASC

The European Arabidopsis Stock Centre

WRIN Collection

Donated by

  • John Mansfield Plant and Microbial Sciences, Division of Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London
  • Alec Forsyth Department of Geographical and Life Sciences, Canterbury Christ Church University
  • N Grabov Department of Geographical and Life Sciences, Canterbury Christ Church University

Click here to view all 301 of these lines.

Description

A collection of 301 WRIN lines (recombinant inbred lines from the Ws-3, Nd-1 cross) used in the study of variation in resistance to the bean pathogen Pseudomonas syringae pv. Phaseolicola in Arabidopsis accessions.

Abstract from paper relating to these stocks

Genetic Dissection of Basal Resistance to Pseudomonas syringae pv. phaseolicola in Accessions of Arabidopsis

We have examined the genetics of nonhost resistance in Arabidopsis, using the bean pathogen Pseudomonas syringae pv. phaseolicola race 6 1448A to probe accessions for natural variation in basal defense. Symptoms rarely developed in leaves of Niedersenz (Nd), some yellowing and occasional necrosis developed in Columbia (Col), whereas tissue collapse was observed in Wassilewskija (Ws) after inoculation by infiltration. Analysis of F2 progeny and recombinant inbred lines (RIL) from a cross between Col and Nd revealed a pattern of continuous symptom increase, indicating the operation of quantitative determinants of resistance. By mapping quantitative trait loci (QTL), significant linkage was determined for resistance (low symptom score) to markers on chromosome 4. Segregation in the F2 cross from Nd × Ws indicated the operation of two dominant genes for resistance, one of which was FLS2 encoding the flagellin receptor. The requirement for FLS2 to confer resistance was confirmed by transgenic experiments, and we showed that the response to P. syringae pv. Phaseolicola was affected by FLS2 gene dosage. Using RIL, the second locus was mapped as a QTL to a large interval on chromosome 1. Both FLS2 and the QTL on chromosome 1 were required for the highest level of resistance to bacterial colonization and symptom development in Nd.

Alec Forsyth1, John W. Mansfield1, Nina Grabov1, Marta de Torres2, Eva Sinapidou3, and Murray R. Grant2

1Division of Biology, Imperial College London, London, SW7 2AZ, U.K.; 2School of Biosciences, University of Exeter, Exeter, EX4 4QD, U.K.; 3NAGREF, Institute of Plant Protection, Thermi 570 01, Thessaloniki, Greece

MPMI Vol. 23, No. 12, 2010, pp. 1545–1552. doi:10.1094 /MPMI -02-10-0047. © 2010 The American Phytopathological Society.

Sets in this collection

These lines are available to individually or in a complete set of 301 lines (N2104806).

Nasc code Description Stock contents
N2104806 Set of recombinant inbred lines from Nd-1 and Ws-3 produced by selfing single progeny of original F2 take to F10. produced N Grabov, A Forsyth, J Mansfield View set contents

References

  • Forsyth, A. et al. 2010. Genetic dissection of basal resistance to Pseudomonas syringae pv. phaseolicola in accessions of Arabidopsis. Molecular plant-microbe interactions:MPMI 23(12):1545-52.PubMed ID: 20653411.