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NASC

The European Arabidopsis Stock Centre

Ehrhardt fused GFP lines

Donated by

  • Sean Cutler Chemical Genomics and Plant Cell Biology, Department of Botany and Plant Sciences, College of Natural and Agricultural Sciences, University of California, Riverside
  • David Ehrhardt Department of Plant Biology, The Carnegie Institution of Washington
  • Chris Somerville Department of Plant Biology, The Carnegie Institution of Washington

Click here to view all 108 of these lines.

Description

Random GFP::cDNA fusion T-DNA lines

These lines were donated by David Ehrhardt, Sean Cutler and Chris Somerville. They are T-DNA lines for which the transforming vector (pEGAD) includes a CaMV 35S promoter-driven GFP coding region fused to random clones from an Arabidopsis cDNA library. The resulting lines express GFP in varying patterns as influenced by the localization/targeting effects of the gene products of the various cDNAs.

A description of pEGAD and a gallery of images documenting the various patterns of GFP expression among the lines is available here.

Ordering

There are 108 lines, each a product of an independent transformation event using the vector library, which may be ordered as a complete set. In addition, a "Basic Set" of 16 lines has been organized on the basis of varying, patterns of GFP expression. Details are in the table below.

Sets in this collection

Nasc code Description Set contents
N84722 Full set of 108 GFP fusion lines; the parental line is included with the set. View set contents
N84723 Basic set of 16 GFP fusion lines; the parental line is included with the set. View set contents
N84724 Set of 92 GFP fusion lines not included in the basic set; the parental line is included with the set. View set contents

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