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NASC

The European Arabidopsis Stock Centre

Jack T-DNA Enhancer Trap Lines

Donated by

  • Tom Jack Department of Biological Sciences, Dartmouth College

Click here to view all 1244 of these lines.

Description

Sets in this collection

Six sets of Jack T-DNA Enhancer trap lines are available as detailed in the table below. Each set is available as pools of 10 or pools of 100. The complete set Stock Number and set contents are shown alongside.

Nasc code Description Set contents
N19650 1st set of 500 pools of 10 Jack T-DNA Promoter trap lines View set contents
N19651 1st set of 50 pools of 100 Jack T-DNA Promoter trap lines View set contents
N31084 2nd set of 630 pools of 10 Jack T-DNA Promoter trap lines View set contents
N31085 2nd set of 63 pools of 100 Jack T-DNA Promoter trap lines View set contents
N31086 Complete set of 1130 pools of 10 Jack T-DNA Promoter trap lines View set contents
N31087 Complete set of 113 pools of 100 Jack T-DNA Promoter trap lines View set contents

Agrobacterium transformant pools of enhancer trap lines

The Thomas Jack laboratory, Dartmouth College, New Hampshire, has donated T-DNA lines and pools which were produced in a glabrous Columbia background (Col-6, gl1-1). A modified vacuum infiltration procedure was utilized to generate this material. The populations were transformed with the pD991 vector which contains an enhancer trap construct and a constitutively expressed NPTII/kanamycin resistance gene between the T-DNA left and right borders. The enhancer-trap construction is comprised of a bacterial uidA encoding beta-glucuronidase (GUS) flanked by -60 minimal Cauliflower Mosaic Virus (CaMV) promoter segment and the 3' untranslated region of the nopaline synthase gene (3' NOS).

The pD991 vector carrying the T-DNA enhancer trap was constructed by cloning an Asp718/BamHI fragment (carrying the -60 CaMV/GUS fusion) from plasmid pD979 into Agrobacterium transformation vector pCGN1547.

For lines numbered 1-2600, inflorescences were stained from individual plants and seeds were collected individually from these plants. For lines numbered 2600-4699, individual plants were stained and seeds collected from pools of 10 plants. For lines numbered higher than 4700, inflorescences were stained and seeds collected in pools of 10 plants.

The distributed T-DNA pools are T4 seeds produced as follows. T1 plants were vacuum-infiltrated with the Agrobacterium culture, and the plants grown to maturity. The kanamycin-resistant T2 seeds were grown, 10 per pot, to maturity. Each resulting pool of T3 seeds was harvested as a group and transmitted to ABRC. The only T-DNA lines which were withheld from the donation were a small number having specific GUS patterns in the inflorescence. No other selections were conducted. Populations consisting of at least 400 T3 plants for each pool of 10 lines were grown at ABRC, and the resulting T4 pools of seeds were harvested and maintained as a single stock.

The seed pools of these lines are distributed primarily as pools of 100 plants, generated by combining the seeds from the above pools (i.e., 10 pools of 10 lines produce a single pool of 100).

NOTE: The individual lines for approximately the first 2000 lines are possessed by ABRC, and are available on a limited basis, by special arrangement and fee. The same is true of all of the pools of 10 seed lines.

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