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NASC

The European Arabidopsis Stock Centre

Soll and Johnson Ds/Ac

Donated by

  • Dieter Soll Department of Chemistry, Graduate School of Arts and Sciences, Yale University
  • Karin Johnson Department of Chemistry, Graduate School of Arts and Sciences, Yale University

Click here to view all 173 of these lines.

Description

Ds Transposon tagged lines

These lines were produced and donated by Dr. Dieter Soll and Dr. Karin Johnson, Yale University, Connecticut. Crosses between two Ac and four Ds parents were made and progeny were grown by self fertilization to the F3 or F4 generation. The Ac parents in the crosses carried a linked GUS gene, and the Ds lines contained streptomycin resistance (StrR) and hygromycin resistance (HmR). The parental lines were produced by Drs. C. Dean and I. Bancroft. Selection for both resistances was practiced in both the F2 and F3 generations. No selection for or against Ac was practiced. The donated stocks consisted of individual F3 or F4 families. A single letter code of the donors' stock number indicates the pedigree, according to the following table.

Ac ParentDs Parent Code
AcGUS1 DsA3 N
AcGUS1 DsB1 P
AcGUS1 DsC12b Q
AcGUS1 DsE T
AcGUS4 DsA3 U
AcGUS4 DsB1 W
AcGUS4 DsC12b Y
AcGUS4 DsE Z

Each of the total 164 lines was independently derived. The genetic background is Landsberg erecta (Ler).

Sets in this collection

Nasc code Description Stock Page
N8995 Complete set of 164 lines, including the parental line N20 (Ler) View set contents

References

  • Bancroft, I. et al. 1992. Development of an efficient two-element transposon tagging system in Arabidopsis thaliana. Molecular & general genetics 233(3):449-61.PubMed ID: 1320189.