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NASC

The European Arabidopsis Stock Centre

Weigel T-DNA lines

Donated by

  • Detlef Weigel Department of Molecular Biology, Max-Planck-Institute for Developmental Biology

Click here to view all 1975 of these lines.

Description

Activation tagged T-DNA pools from Dr. Detlef Weigel

Dr. Detlef Weigel, The Salk Institute for Biological Sciences, La Jolla, California generated activation-tag T-DNA lines. Initially, 850 of these lines were donated directly by Dr. Weigel, and a subsequent 7700 were grown to distribution-level quantities by Dr. Michael Sussman, Dept. of Horticulture, University of Wisconsin, Madison. These constitute Set 1 of the Weigel lines. A further 7848 lines are available and constitute Set 2 of the Weigel lines. Information on distribution of both sets are given below.

The background for this population is Columbia (Col-7). The pSKI15 vector used in the transformations contains an activation tag construct, a pBstKS+ plasmid rescue sequence and a phosphonothricin resistance gene (BAR driven by 5' MAS) between the T-DNA left and right borders. The activation-tag construction is comprised of multimerized 35S Cauliflower Mosaic Virus (CaMV) enhancers. Plasmid rescue can be conducted in conjunction with either the right or left T-DNA borders. See the Weigel web site for details of the rescue procedure and the sequence of the T-DNA. The T-DNA sequence is also available on the TAIR site. The distributed T-DNA pools are T4 seeds.

Distribution of Weigel T-DNA lines

There are now three sets of Weigel Lines distributed by NASC. These sets are distributed differently as described below.

Set 1

The seed pools of these lines are distributed primarily as pools of 100 lines. For the first 850 lines, pools of 10 and single lines are available. The remaining 7,700 lines are available only as pools of 20. Stock numbers and costs are summarised below.

Stock # Description Price (£)
N21995 86 pools of 100 (1st and 2nd donations) 107
N21999 Set of 85 pools of 10 lines (1st donation, 850 lines) 95
N21998 Set of 385 pools of 20 lines (2nd donation, 7700 lines) 372

NOTE: All sets of pools include Col-7 wild type (N3731).

Set 2

The seed pools of these lines are distributed as 82 pools of 96. The complete set can be ordered as N21991. 8 pools of 12 make up a pool of 96 lines, and in total 654 pools of 12 are available. (See the Weigel stock page for further information). Please check with us for availability information regarding a complete set of the pools of 12.

Stock # Description Price (£)
N21991 Set of 82 pools of 96 lines 107
N21992 Set of 654 pools of 12 lines 961

Set 3

Stock # Description Price (£)
N23153 Set of 62 pools of 100 lines 82
N23154 Set of 621 pools of 10 lines 811

Sets in this collection

Nasc code Description Stock contents
N21991 96s Set 2 - Set of 82 pools of 96 lines View set contents
N21992 12s Set 2 - Set of 654 pools of 12 lines View set contents
N21995 100s Set 1 - 86 pools of 100 (1st and 2nd donations) View set contents
N21996 100s Set 1 - 77 pools of 100 View set contents
N21997 100s Set 1 - 9 pools of 100 View set contents
N21998 20s Set 1 - Set of 385 pools of 20 lines (2nd donation, 7700 lines) View set contents
N21999 10s Set 1 - Set of 85 pools of 10 lines (1st donation, 850 lines) View set contents
N23153 100s Set 3 - Set of 62 pools of 100 lines View set contents
N23154 10s Set 3 - Set of 621 pools of 10 lines View set contents

Related links

References

  • Weigel, D. et al. 2000. Activation tagging in Arabidopsis. Plant Physiology 122(4):1003-13. PMID. 10759496.