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The European Arabidopsis Stock Centre

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Search result for '122 '. Viewing records 1 to 9 of 9 hits.



N9981 Name: Noccaea caerulescens Saint Laurent Le Minier A1M4 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
5500 M0 seeds were irradiated with fast neutrons for 137 min, at a dose rate of 60 Gy (Biological Irradiation Facility, Budapest Research Reactor (BRR), Budapest Neutron Centre, Budapest, Hungary). The mean water kerma dose rate was monitored in real time by U-235 and Th-232 fission chambers and a Geiger–Mu¨ller counter and was 438 mGy min-1 (± 3.0%). The BRR tank-type reactor, with a nominal thermal power of 10 MW, was moderated and cooled by light water. Filters of different densities were arranged in order to decrease gamma and neutron intensity and to modify the neutron spectrum and the neutron : gamma ratio. The filters were set at the following distances from the core towards the irradiation cavity: 143.6 mm Al + 18 mm Pb + 15 mm Al. At the beam stop behind the sample, shielding comprised 30 mm Fe + 45 mm Pb + 8 mm Al + 20 mm B4C. Seeds were irradiated inside a rotating Cd capsule (16 rpm) of 2 mm wall thickness at a temperature < 30_C and humidity < 70%, at normal air pressure. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost. After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. Following vernalization of M1 plants, 79% of plants survived to maturity. In all, 80 000 M2 lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with A1 flowering in < 12 wk and selfed to the M4 without vernalization.
Phenotype
Early Flowering (within 2 months) and early fruiting (between 3-4 months) non vernal obligate mutant lines, selfed to M4
N9982 Name: Noccaea caerulescens Saint Laurent Le Minier A2M5 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
5500 M0 seeds were irradiated with fast neutrons for 137 min, at a dose rate of 60 Gy (Biological Irradiation Facility, Budapest Research Reactor (BRR), Budapest Neutron Centre, Budapest, Hungary). The mean water kerma dose rate was monitored in real time by U-235 and Th-232 fission chambers and a Geiger–Mu¨ller counter and was 438 mGy min-1(± 3.0%). The BRR tank-type reactor, with a nominal thermal power of 10 MW, was moderated and cooled by light water. Filters of different densities were arranged in order to decrease gamma and neutron intensity and to modify the neutron spectrum and the neutron : gamma ratio. The filters were set at the following distances from the core towards the irradiation cavity: 143.6 mm Al + 18 mm Pb + 15 mm Al. At the beam stop behind the sample, shielding comprised 30 mm Fe + 45 mm Pb + 8 mm Al + 20 mm B4C. Seeds were irradiated inside a rotating Cd capsule (16 rpm) of 2 mm wall thickness at a temperature < 30_C and humidity < 70%, at normal air pressure. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost. After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. Following vernalization of M1 plants, 79% of plants survived to maturity. In all, 80 000 M2 lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with A2 flowering in < 12 wk and selfed to the M5 without vernalization.
Phenotype
Early Flowering (within 2 months) and early fruiting (between 3-4 months) nonvernal obligate mutant lines, selfed to M5
N9983 Name: Noccaea caerulescens Saint Laurent Le Minier A5M4 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
5500 M0 seeds were irradiated with fast neutrons for 137 min, at a dose rate of 60 Gy (Biological Irradiation Facility, Budapest Research Reactor (BRR), Budapest Neutron Centre, Budapest, Hungary). The mean water kerma dose rate was monitored in real time by U-235 and Th-232 fission chambers and a Geiger–Mu¨ller counter and was 438 mGy min-1 (± 3.0%). The BRR tank-type reactor, with a nominal thermal power of 10 MW, was moderated and cooled by light water. Filters of different densities were arranged in order to decrease gamma and neutron intensity and to modify the neutron spectrum and the neutron : gamma ratio. The filters were set at the following distances from the core towards the irradiation cavity: 143.6 mm Al + 18 mm Pb + 15 mm Al. At the beam stop behind the sample, shielding comprised 30 mm Fe + 45 mm Pb + 8 mm Al + 20 mm B4C. Seeds were irradiated inside a rotating Cd capsule (16 rpm) of 2 mm wall thickness at a temperature < 30_C and humidity < 70%, at normal air pressure. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost. After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. Following vernalization of M1 plants, 79% of plants survived to maturity. In all, 80 000 M2 lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with A5 flowering in < 12 wk and selfed to the M4 without vernalization.
Phenotype
Early Flowering (within 2 months) and early fruiting (between 3-4 months) non vernal obligate mutant lines, selfed to M4.
N9984 Name: Noccaea caerulescens Saint Laurent Le Minier A7M5 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
5500 M0 seeds were irradiated with fast neutrons for 137 min, at a dose rate of 60 Gy (Biological Irradiation Facility, Budapest Research Reactor (BRR), Budapest Neutron Centre, Budapest, Hungary). The mean water kerma dose rate was monitored in real time by U-235 and Th-232 fission chambers and a Geiger–Mu¨ller counter and was 438 mGy min-1 (± 3.0%). The BRR tank-type reactor, with a nominal thermal power of 10 MW, was moderated and cooled by light water. Filters of different densities were arranged in order to decrease gamma and neutron intensity and to modify the neutron spectrum and the neutron : gamma ratio. The filters were set at the following distances from the core towards the irradiation cavity: 143.6 mm Al + 18 mm Pb + 15 mm Al. At the beam stop behind the sample, shielding comprised 30 mm Fe + 45 mm Pb + 8 mm Al + 20 mm B4C. Seeds were irradiated inside a rotating Cd capsule (16 rpm) of 2 mm wall thickness at a temperature < 30_C and humidity < 70%, at normal air pressure. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost. After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. Following vernalization of young M1 plants, 79% of plants survived to maturity. In all, 80 000 M2 lines were screened for flowering in the absence of vernalization. Floral initials were observed in 35 lines, with A7 flowering in < 12 wk and selfed to the M5 without vernalization.
Phenotype
Early Flowering (within 2 months) and early fruiting (between 3-4 months) nonvernal obligate mutant lines, selfed to M5
N9985 Name: Saint Laurent Le Minier Nc1 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
Seeds were selected from a wild type accession (Nc1) from a Noccaea caerulescens Saint Laurent Le Minier population and selfed to S3 by single seed descent. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost.
Phenotype
Single seed descent to S3 wild type lines, requiring vernalisation (5°C for 12 weeks) to initiate flowering. Fruiting between 7-9 months.
N9986 Name: Saint Laurent Le Minier Nc3 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
Seeds were selected from a wild type accession (Nc3) from a Noccaea caerulescens Saint Laurent Le Minier population and selfed to S3 by single seed descent. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost.
Phenotype
Single seed descent to S3 wild type lines, requiring vernalisation (5°C for 12 weeks) to initiate flowering. Fruiting between 7-9 months.
N9987 Name: Saint Laurent Le Minier Nc5 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
Seeds were selected from a wild type accession (Nc5) from a Noccaea caerulescens Saint Laurent Le Minier population and selfed to S3 by single seed descent. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost.
Phenotype
Single seed descent to S3 wild type lines, requiring vernalisation (5°C for 12 weeks) to initiate flowering. Fruiting between 7-9 months
N9988 Name: Saint Laurent Le Minier Nc6 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
Seeds were selected from a wild type accession (Nc6) from a Noccaea caerulescens Saint Laurent Le Minier population and selfed to S3 by single seed descent. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost.
Phenotype
Single seed descent to S3 wild type lines, requiring vernalisation (5°C for 12 weeks) to initiate flowering. Fruiting between 7-9 months.
N9989 Name: Saint Laurent Le Minier Nc7 Price: £11.00
Donor
  • University of Nottingham Martin Broadley
  • University of Nottingham Seosamh O'Lochlainn
  • University of Nottingham Neil Graham
Stock type: individual line
Material type: seed


Description
Seeds were selected from a wild type accession (Nc7) from a Noccaea caerulescens Saint Laurent Le Minier population and selfed to S3 by single seed descent. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost.
Phenotype
Single seed descent to S3 wild type lines, requiring vernalisation (5°C for 12 weeks) to initiate flowering. Fruiting between 7-9 months.