NASC

N2106105

Name: SAND S18

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106106

Name: SAND S29

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106107

Name: SAND SUC2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106108

Name: SAND WOL

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106109

Name: SAND SHR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106110

Name: SAND ATHB8

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106111

Name: SAND IAA19

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106112

Name: SAND DR5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus synthetic

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106113

Name: SAND PIN1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106114

Name: SAND PIN4

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106115

Name: SAND PIN7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106116

Name: SAND PIN2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106117

Name: SAND WER

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106118

Name: SAND EXP7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106119

Name: SAND PRP3

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106120

Name: SAND IRT1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106121

Name: SAND GL2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106122

Name: SAND CO2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106123

Name: SAND PEP

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106124

Name: SAND SCR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106125

Name: SAND E47

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106126

Name: SAND UPB1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106127

Name: SAND WOX5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106128

Name: SAND Q12

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106129

Name: SAND FEZ

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106130

Name: SAND UBQ10

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106131

Name: SAND 2x35S

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus Cauliflower mosaic virus promoter

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106132

Name: SAND RCH1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype SAND line. Cell type specific promoter expressing a 4xYFP tag in the cytoplasm

N2106133

Name: SAND KN

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106134

Name: RED TIDE S17

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106135

Name: RED TIDE S18

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106136

Name: RED TIDE S29

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106137

Name: RED TIDE S32

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106138

Name: RED TIDE SUC2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106139

Name: RED TIDE WOL

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106140

Name: RED TIDE SHR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106141

Name: RED TIDE ATHB8

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106142

Name: RED TIDE IAA19

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106143

Name: RED TIDE DR5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus synthetic

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106144

Name: RED TIDE PIN1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106145

Name: RED TIDE PIN4

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106146

Name: RED TIDE WER

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106147

Name: RED TIDE EXP7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106148

Name: RED TIDE PRP3

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106149

Name: RED TIDE IRT1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106150

Name: RED TIDE GL2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106151

Name: RED TIDE CO2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106152

Name: RED TIDE PEP

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106153

Name: RED TIDE SCR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus

N2106154

Name: RED TIDE E47

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus