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The European Arabidopsis Stock Centre

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Search result for '19983 '. Viewing records 1 to 3 of 3 hits.



N68100 Name: pMLO9::GUS Price: £11.00
Donor
  • University of North Carolina, Chapel Hill Alan M. Jones
Stock type: individual line
Material type: seed


Description
DNA fragment (covering 5' upstream of the AtMLO9 promoter region) from Col-0 was amplified and cloned into the SmaI site of pBluescript, then excised as EcoRI/NcoI and co-ligated with an NcoI/HindIII GUS fragment (excised from pSLJ4D4) into the binary vector pPZP211, and the Col-0 plants were transformed using the floral dip method. Homozygous for the transgene. Kanamycin resistant.
Phenotype
Expressed in vascular tissues, petioles of the leaf and cotyledon, stem and in the the floral organs during or after the shedding process.
N68101 Name: pMLO4::GUS Price: £11.00
Donor
  • University of North Carolina, Chapel Hill Alan M. Jones
Stock type: individual line
Material type: seed


Description
DNA fragment (covering 5' upstream of the AtMLO4 promoter region) from Col-0 was amplified and cloned into the SmaI site of pBluescript, then excised as EcoRI/NcoI and co-ligated with an NcoI/HindIII GUS fragment (excised from pSLJ4D4) into the binary vector pPZP211, and the Col-0 plants were transformed using the floral dip method. Homozygous for the transgene. Kanamycin resistant.
Phenotype
Expressed in root tip and vascular tissues, petioles of the leaf and cotyledon, stem and in the the floral organs during or after the shedding process
N70000 Name: Col-0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
line used to generate the current reference genome sequence; re-sequenced by the J. Ecker laboratory using Illumina SBS technology; derived from a bulk-seed descendant of CS60000, which was used to generate the Ecker T-DNA and cDNA collections plus many publicly available mutant lines and clones.
Phenotype
Medium rosette; leaf margins slightly serrated; observed flowering time was 22 days after planting; average height = 32 cm.