Search result for '19990 '.
Viewing records 1 to 11 of 11 hits.
|
N3763
|
Name: KAT1:GUS 29 |
Price:
£11.00
|
Donor
- University of Wisconsin, Madison Michael Sussman
|
|
Stock type: individual line
|
Material type: seed
|
|
Description
KAT1::GUS, contains the promoter KAT1 (K+ transporter Arabidopsis thaliana) fused to the GUS reporter gene; encodes a voltage-gated inward-rectifying potassium channel; expressed primarily in guard cells in hypocotyls, cotyledons and leaves of young plants; dominant gene action; homozygous for the T-DNA.
|
Phenotype
KAT1:GUS (Arabidopsis K+ transporter, line 29 ); dominant; expression of K+ channel gene in guard cells, roots and flowers; transgenic stock.
|
|
N6141
|
Name: KNAT1::GUS #1 |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Sarah Hake
|
|
Stock type: individual line
|
Material type: seed
|
|
Phenotype
contains KNAT1::GUS -1, iudA reporter gene (B-glucuronidase (GUS) driven by the KNAT1 (knotted-like of Arabidopsis thaliana) promoter; expression was detected in the hypocotyl, stem and shoot meristems but not in leaves.
|
|
|
N6357
|
Name: Col-0:PR1/GUS |
Price:
£11.00
|
Donor
- University of Delaware Allan Shapiro
- University of Delaware Chu Zhang
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Transcriptional reporter construct that accurately reflects pathogenesis-related (PR1) transcription with beta-glucuronidase (GUS) as a reporter gene ie. PR1 entire coding region:GUS
|
Phenotype
no visible phenotype.
|
|
N6497
|
Name: CPC::GUS |
Price:
£11.00
|
Donor
- RIKEN Takuji Wada
- Kyoto University Kiyotaka Okada
- Kyoto University Yoshihiro Kimura
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
pCPC::GUS; for the construction of the CPC promoter::GUS, a PstI-Bbs I fragment of the CPC (CAPRICE) promoter was fused to the GUS reporter gene and was subcloned into pBI101-Hm3 binary vector, this plasmid was introduced into an Agrobacterium tumefaciens strain C58::pGV2260; plant transformation was performed by a vacuum infiltration procedure; no visible morphological phenotype; kanamycin resistant.
|
|
|
N8849
|
Name: LFY::GUS |
Price:
£11.00
|
Donor
- Mendel Biotechnology Inc. Frederick Hempel
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
LFY::GUS, contains pDW150 derived from pDW137, a pCGN1547 derivative containing the HindIII-EcoRI fragment of pBI101.2, which encompasses the B-glucuronidase (GUS) coding region and the nos terminator, the 2.3 kb BamHI fragment spanning the LFY promoter was inserted in front of GUS in pDW137, such that the fusion gene uses the LFY initiation codon, which overlaps the downstream BamHI site; LFY expression detected before floral determination, with gradual changes during the vegetative phase of plant development, changing quantitatively during the photoinduced transition to flowering
|
|
|
N8851
|
Name: GL2:GUS |
Price:
£11.00
|
Donor
- University of Minnesota David Marks
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
GL2::GUS; carries a GUS coding sequence controlled by a glabrous2 promoter; expressed in shoots, involving spatial and temporal variation in developing leaves and trichomes; expressed in developing trichomes and in cells surrounding trichomes during early stages of trichome development persisting in mature trichomes.
|
Phenotype
GUS expressed in shoots, involving spatial and temporal variation in developing leaves and trichomes; also expressed in developing trichomes and in cells surrounding trichomes during early stages of trichome development persisting in mature trichomes.
|
|
N16358
|
Name: GA3ox3-TL-GUS |
Price:
£11.00
|
Donor
- Duke University Tai-ping Sun
|
|
Stock type: individual line
|
Material type: seed
|
|
Description
Transgenic GUS-fusion expression line # 14-2-11, harboring the GA3ox3-TL-GUS construct. The promoter- á -glucuronidase (GUS) translational fusion (/TL-GUS/) was generated using the entire intergenic sequence upstream of GA3ox3 including an intron-containing 5'-segment of the coding region fused to GUS; final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants were selected with kanamycin.
|
Phenotype
Strong expression in anthers and developing embryos.
|
|
N25261
|
Name: ARR5:GUS |
Price:
£11.00
|
Donor
- University of North Carolina, Chapel Hill Joe Kieber
|
|
Stock type: individual line
|
Material type: seed
|
|
Description
Transgenic line harboring the ARR5 promoter fused to the beta-glucuronidase (GUS) reporter gene; kanamycin resistant
|
Phenotype
High increase in reporter activity in response to cytokinin; primarily expressed in the root and shoot meristems in the absence of exogenous cytokinin; in the presence of 10 nM benzyladenine (BA), the expression region is enlarged to include tissues around the shoot meristematic region; strong expression is induced in all tissues in the root, from the hypocotyl-root junction through the root tip.
|
|
N25262
|
Name: ARR6:GUS |
Price:
£11.00
|
Donor
- University of North Carolina, Chapel Hill Joe Kieber
- Dartmouth College Eric Schaller
- University of South Carolina Beth Krizek
- University of California, San Diego Nigel Crawford
- University of California, Davis Luca Comai
- University of Utah Gary Drews
- Universitat Tubingen John M. Ward
- Oregon State University James Atland
- University of Puget Sound Andreaas Madlung
|
|
Stock type: individual line
|
Material type: seed
|
|
Description
Transgenic line harboring the ARR6 promoter fused to the §-glucuronidase (GUS) reporter gene; kanamycin resistant; High increase in reporter activity in response to cytokinin; expression was detected in the shoot meristematic region and cotyledon vasculature; cytokinin treatment resulted in overall higher levels of expression, with GUS staining expanded to the hypocotyl and root tissues but excluded from the root tip.. High increase in reporter activity in response to cytokinin; expression was detected in the shoot meristematic region and cotyledon vasculature; cytokinin treatment resulted in overall higher levels of expression, with GUS staining expanded to the hypocotyl and root tissues but excluded from the root tip..
|
|
|
N70756
|
Name: ProCESA2:GUS |
Price:
£11.00
|
Donor
- University of California, Davis Deborah Delmer
|
|
Stock type: individual line
|
Material type: seed
|
|
Description
ProCESA2::GUS, transgenic line containing the cellulose synthase 2 (CESA2) promoter, E. coli B-glucuronidase (GUS) reporter gene; genomic fragments upstream of the CESA initiating AUG codon were amplified by PCR, cloned into pRITA upstream of the GUS gene, and sequenced to confirm accuracy of amplification; the promoter:GUS cassettes were then cloned at NotI fragments into the Ti plasmid pMLBART in the same orientation as the BAR gene, introduced into Agrobacterium strain ASE1 by electroporation, and used to transform Col-0 Arabidopsis by the floral dip method; transgenic plants were selected with a 1:1,000 dilution of Finale (Hoechst-Roussel Agri-Vet, Somerville, NJ) twice a week for three weeks; expression of GUS was confirmed by the standard histological assay at several subsequent generations.
|
Phenotype
ProCESA2 highly expressed in roots, rosette, cauline leaves, stems and flowers; expression is slightly toward zones that are less rapidly expanding.
|
|
N70764
|
Name: ProCESA10:GUS |
Price:
£11.00
|
Donor
- University of California, Davis Deborah Delmer
|
|
Stock type: individual line
|
Material type: seed
|
|
Description
ProCESA10::GUS, transgenic line containing the cellulose synthase 10 (CESA10) promoter, E. coli B-glucuronidase (GUS) reporter gene; genomic fragments upstream of the CESA initiating AUG codon were amplified by PCR, cloned into pRITA upstream of the GUS gene, and sequenced to confirm accuracy of amplification; the promoter:GUS cassettes were then cloned at NotI fragments into the Ti plasmid pMLBART in the same orientation as the BAR gene, introduced into Agrobacterium strain ASE1 by electroporation, and used to transform Col-0 Arabidopsis by the floral dip method; transgenic plants were selected with a 1:1,000 dilution of Finale (Hoechst-Roussel Agri-Vet, Somerville, NJ) twice a week for three weeks; expression of GUS was confirmed by the standard histological assay at several subsequent generations.
|
Phenotype
CESA10::GUS transgene expression initially detected in ovules.
|
