Home | About NASC | Address/Staff
Ask a question | Help

NASC

Please note that due to the global coronavirus epidemic orders placed with NASC may take longer than usual to reach you

NASC Stock Detail Page

NASC ID: N16269

Name: pgp4-1

ABRC stock number: CS16269

Description: Confirmed line isolated from original SALK line SALK_063720; homozygous for the insertion. Selected after PCR analysis of the insertion site. PGP4 exhibits apolar plasma membrane localization in the root cap and polar localization in tissues above. Auxin influx transporter shows reversibility in heterologous systems.

Donation Date: 2007-09-19

Donated by:  Purdue University Peter Goldsbrough
Purdue University Angus Murphy

Donor Number: pgp4-1

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Comment: recessive

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000, SALK_063720)

Segregation status: homozygous

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • pgp4-1
  • pgp

Phenotype

Reduced root gravitropic bending and elongation as well as lateral root formation; reduced root elongation is conditional and dependent on light intensity and sucrose. Reduced basipetal auxin transport in roots (~35%) and a small decrease in shoot-to-root transport consistent with a partial loss of the redirective auxin sink in the root.

References

Terasaka, K. et al. 2005. PGP4, an ATP binding cassette P-glycoprotein, catalyzes auxin transport in Arabidopsis thaliana roots. The Plant cell 17(11):2922-39.PubMed ID: 16243904.