NASC Stock Detail Page
NASC ID: N2106367
Name: SAND Seed Set
ABRC stock number: CS2106367
Description: Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
Donation Date: 2015-10-29
| Donated by: | Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert |
Stock type: set (View set contents)
Material type: seed
Comment: Donor names: Yvon Jaillais, Gregory Vert.
| Status | Price (£) |
|---|---|
| Available | £58.00 |
Germplasm Info
Mutagen: ema
Background: Col
References
Mar Marquès-Bueno, M. et al. 2016. A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell-type specific functional genomics in Arabidopsis. The Plant Journal 85(2):320-33. PubMed ID: 26662936.