NASC Stock Detail Page
NASC ID: N25435
Name: cax1-1
ABRC stock number: CS25435
Donation Date: 2006-10-16
| Donated by: | Baylor College of Medicine Kendal Hirschi |
Donor Number: cax1-1
Part of : Homozygous lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transposon insertion
Mutagen: transposon insertion
Background: Col-4 (N933)
Segregation status: homozygous
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Vacuolar H+/Ca2+ transporter; play a central role in Ca2+ and metal sequestration into the vacuole, localized in the tonoplast; predominately expressed in leaves. Altered plant development: alterations in lateral root growth (seedlings display 11% reduction in primary root length when grown on normal medium, number of lateral roots is reduced by 25%, length of the lateral roots is reduced by almost 50%); alterations in apical dominance (reduced branching, reduced length of the secondary inflorescences); delayed flowering; reduced plant height; eventually plants grow and produce seeds normally. Enhanced growth under Mn2+ and Mg2+ stress conditions; perturbed hormone sensitivities (roots are resistant to auxin but are tolerant to 1-aminocycloproprane-1-carboxylic acid (an ethylene precursor), abscisic acid, and benzyladenine; sensitive to gibberellic acid and epibrassinolide); modestly sensitive to exogenous Ca2+; exhibit a 50% reduction in tonoplast Ca2+/H+ antiport activity, a 40% reduction in tonoplast V-type H+-translocating ATPase activity, a 36% increase in tonoplast Ca2+-ATPase activity; high capacity transporter responsible for maintaining low cytosolic-free Ca2+ concentrations in plant cells by catalyzing pH gradient-energized vacuolar Ca2+ accumulation."
References
Cheng, N.H., et al. 2003. The Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development, and hormonal responses and reveals interplay among vacuolar transporters. The Plant Cell 15(2):347-64.PubMed ID: 12566577.