NASC Stock Detail Page
NASC ID: N530155
Name: SALK_030155
ABRC stock number: SALK_030155
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_030155.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G51880.1 (at chr 5 pos 21089239 (W/21089174-21089239) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 4, R 1, C 5 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO370970. SALKseq_030155.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G77640.1=UTR3 and AT1G77650.1 (at chr 1 pos 29179980 (W/29179941-29179980) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 4, R 1, C 5 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO357568. SALKseq_030155.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G78510.1 (at chr 1 pos 29535784 (C/29535784-29535865) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 4, R 1, C 5 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO360913. SALKseq_030155.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G32930.1 (at chr 2 pos 13966433 (W/13966390-13966433) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 4, R 1, C 5 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364271. SALKseq_030155.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G35350.1 and AT2G35360.1=UTR3 (at chr 2 pos 14887993 (C/14887993-14888051) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 4, R 1, C 5 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO367616.
Donation Date: 2002-01-02
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_030155
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_030155C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At5g51880 MJM18.3 L300-5'
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.