NASC

NASC ID: N530578

Name: SALK_030578

ABRC stock number: SALK_030578

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_030578.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G22130.1 (at chr 5 pos 7339672 (W/7339640-7339672) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 49-77 bps onto the T-DNA. Pools P 5, R 3, C 7 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO407784.

SALKseq_030578.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G24915 and AT5G24920.1 (at chr 5 pos 8584392 (W/8584341-8584392) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 47-75 bps onto the T-DNA. Pools P 5, R 3, C 7 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411130.

SALKseq_030578.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G80370.1 (at chr 1 pos 30215948 (W/30215870-30215948) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 5, R 3, C 7 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO397770.

SALKseq_030578.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G60400.1 (at chr 3 pos 22330334 (W/22330281-22330334) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 5, R 3, C 7 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401111.

SALKseq_030578.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G65110.1 (at chr 5 pos 26011227 (W/26011164-26011227) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 5, R 3, C 7 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO404448.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_030578

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664516 (SALK_030578C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		PNT1	AT1G80370 AT3G60400 AT5G24915 AT5G24920 AT5G65110 At5g22130

EMBL accession:

BH749962

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.