NASC Stock Detail Page
NASC ID: N532851
Name: SALK_032851
ABRC stock number: SALK_032851
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_032851.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G21465.1 (at chr 3 pos 7562876 (W/7562841-7562876) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 7, R 5, C 10 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO347684. SALKseq_032851.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G01180.1=P355 and AT2G01190.1=P355 (at chr 2 pos 113407 (W/113347-113407) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 7, R 5, C 10 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO340984. SALKseq_032851.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G50350.1 (at chr 5 pos 20498497 (W/20498420-20498497) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 7, R 5, C 10 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351036. SALKseq_032851.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28923 and AT3G28925.1 (at chr 3 pos 10957858 (C/10957858-10957919) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 7, R 5, C 10 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344341.
Donation Date: 2001-01-12
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_032851
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N657578 (SALK_032851C).
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.