NASC

NASC ID: N533203

Name: SALK_033203

ABRC stock number: SALK_033203

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_033203.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G58760.1 (at chr 3 pos 21729611 (W/21729533-21729611) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 7, R 2, C 10 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411257.

SALKseq_033203.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G05710.1 (at chr 2 pos 2145678 (W/2145632-2145678) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 7, R 2, C 10 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO407908.

SALKseq_033203.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT4G25260.1 (at chr 4 pos 12935970 (C/12935970-12936023) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 52-77 bps onto the T-DNA. Pools P 7, R 2, C 10 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO341001.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_033203

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662046 (SALK_033203C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G05710 At4g25260 At3g58760

EMBL accession:

ED580247

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At3g58760

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.