NASC

NASC ID: N533634

Name: SALK_033634

ABRC stock number: SALK_033634

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_033634.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G18370.1 (at chr 3 pos 6306165 (W/6306105-6306165) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools: P 9, R 1, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.
SALKseq_033634.1 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G13210.1 (at chr 3 pos 4246629 (C/4246629-4246707) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools: P 9, R 1, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.
SALKseq_033634.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G53140.1 (at chr 5 pos 21552197 (W/21552147-21552197) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 46-69 bps onto the T-DNA. Pools: P 9, R 1, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.
SALKseq_033634.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G07700.1 (around chr 5 pos 2450580 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.
SALKseq_033634.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G61420.2 (at chr 5 pos 24690607 (W/24690551-24690607) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools: P 9, R 1, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_033634

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665045 (SALK_033634C ).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G13210 AT5G07700 AT5G41770 AT5G53140 AT5G61420 At3g18370

EMBL accession:

ED580528

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At3g18370

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.