NASC

NASC ID: N536661

Name: SALK_036661

ABRC stock number: SALK_036661

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At5g58370. MCK7.24 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_036661.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G58370.1 (at chr 5 pos 23594138 (C/23594138-23594183) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 8, R 9, C 7 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO384661.

SALKseq_036661.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G27820.1=UTR3 and AT2G27830.1=P355 (at chr 2 pos 11859769 (W/11859719-11859769) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 8, R 9, C 7 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371268.

SALKseq_036661.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G48560.1 (at chr 3 pos 18003778 (C/18003778-18003856) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 8, R 9, C 7 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO374618.

SALKseq_036661.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G48740.1=P355 and AT3G48745=P355 (at chr 3 pos 18068701 (W/18068640-18068701) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 8, R 9, C 7 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO377965.

SALKseq_036661.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G42950.1 (at chr 5 pos 17231016 (W/17230952-17231016) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 8, R 9, C 7 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381303.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_036661

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662116 (SALK_036661C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G27820 AT2G27830 AT3G48560 AT3G48740 AT3G48745 AT5G42950 At5g58370

EMBL accession:

ED584666

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.