NASC

NASC ID: N538326

Name: SALK_038326

ABRC stock number: SALK_038326

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_038326.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G35950 and AT1G35960.1 (around chr 1 pos 13388987 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414852.

SALKseq_038326.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G09170.1 (at chr 4 pos 5844056 (C/5844056-5844115) on the TAIR10). The sequences' 20-79 bps mapped on the genome, 1-19 bps onto the T-DNA. Pools P 10, R 4, C 10 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408151.

SALKseq_038326.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G60210.2=P355 and AT5G60220.1 (at chr 5 pos 24248128 (W/24248088-24248128) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 38-55 bps onto the T-DNA. Pools P 10, R 4, C 10 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411500.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_038326

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665029 (SALK_038326C ).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G35950 AT1G35960 AT4G09170 AT5G60210 AT5G60220 At3g14290

EMBL accession:

CC052808

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.