NASC

NASC ID: N538594

Name: SALK_038594

ABRC stock number: SALK_038594

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_038594.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G24860.1 (at chr 3 pos 9074012 (W/9073959-9074012) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 11, R 12, C 16 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO394820.

SALKseq_038594.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G18042 and AT2G18050.2=P355 (at chr 2 pos 7845271 (W/7845194-7845271) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 11, R 12, C 16 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388126.

SALKseq_038594.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G50140.1=P355 and AT3G50150.1=UTR3 (at chr 3 pos 18595127 (C/18595127-18595189) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 11, R 12, C 16 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391463.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_038594

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665805 (SALK_038594C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G18042 AT2G18050 At3g24860 AT3G50140 AT3G50150

EMBL accession:

BH907179

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.