NASC

NASC ID: N538916

Name: SALK_038916

ABRC stock number: SALK_038916

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_038916.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G67600.1 (at chr 5 pos 26959868 (W/26959806-26959868) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 10, R 5, C 1 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351336.

SALKseq_038916.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G46850.1 (at chr 2 pos 19250791 (C/19250791-19250837) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 36-74 bps onto the T-DNA. Pools P 10, R 5, C 1 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411535.

SALKseq_038916.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G51820.1 (at chr 3 pos 19216663 (C/19216663-19216730) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 10, R 5, C 1 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO341280.

SALKseq_038916.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G01850.1 (at chr 4 pos 797698 (W/797648-797698) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 10, R 5, C 1 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344636.

SALKseq_038916.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G50490.1 (at chr 5 pos 20561312 (C/20561312-20561355) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 10, R 5, C 1 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO347986.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_038916

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N658196 (SALK_038916C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G46850 AT3G51820 AT4G01850 AT5G50490 At5g67600

EMBL accession:

ED583871

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g67600

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.