NASC

NASC ID: N539033

Name: SALK_039033

ABRC stock number: SALK_039033

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_039033.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G22870.1 (at chr 1 pos 8090302 (W/8090248-8090302) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 15, R 18, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO398182.

SALKseq_039033.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G61898.1=UTR3 and AT3G61900.1 (at chr 3 pos 22923516 (W/22923457-22923516) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 15, R 18, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401525.

SALKseq_039033.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G46025.1 and AT5G46030.1=P355 (at chr 5 pos 18666566 (C/18666566-18666644) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 15, R 18, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO404851.

SALKseq_039033.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G54830.1 (at chr 5 pos 22272960 (C/22272960-22273038) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 15, R 18, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408193.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_039033

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665809 (SALK_039033C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G61898 AT3G61900 AT5G46025 AT5G46030 AT5G54830 At1g22870

EMBL accession:

ED583952

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g22870

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.