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The European Arabidopsis Stock Centre

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NASC ID: N542504

Name: SALK_042504

ABRC stock number: SALK_042504

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2003 TAIR gene annotation -At1g79380 T8K14.20 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_042504.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G79380.1 (at chr 1 pos 29861058 (W/29861001-29861058) on the TAIR10). The sequences' 2-59 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 14, R 18, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344794.

SALKseq_042504.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G37150.2 (at chr 1 pos 14176864 (W/14176786-14176864) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 14, R 18, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO341439.

SALKseq_042504.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G18300.1 (at chr 3 pos 6283099 (W/6283054-6283099) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 39-61 bps onto the T-DNA. Pools P 14, R 18, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO348147.

SALKseq_042504.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G24590.1 (at chr 3 pos 8970650 (C/8970650-8970714) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 14, R 18, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351495.

SALKseq_042504.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G27010.1 (at chr 4 pos 13567888 (W/13567826-13567888) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 14, R 18, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354831.

Donation Date: 2003-04-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_042504

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N657373 (SALK_042504C).

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.