NASC

NASC ID: N542677

Name: SALK_042677

ABRC stock number: SALK_042677

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_042677.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G64420 (around chr 1 pos 23933136 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO415001.

SALKseq_042677.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G14480.1 (at chr 4 pos 8331486 (C/8331486-8331536) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 47-77 bps onto the T-DNA. Pools P 16, R 16, C 12 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371569.

SALKseq_042677.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G08075 (at chr 5 pos 2588035 (W/2587977-2588035) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 16, R 16, C 12 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO374923.

SALKseq_042677.6 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G03350.1 (at chr 5 pos 816652 (W/816590-816652) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 16, R 16, C 12 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378268.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_042677

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_042677C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G64420 AT4G14480 AT5G03350 AT5G08075 At3g46540

EMBL accession:

ED586865

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g46540

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.