NASC

NASC ID: N544326

Name: SALK_044326

ABRC stock number: SALK_044326

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: F12E4.280 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g03500.

Associated Polymorphisms:

SALKseq_044326.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G03495.1 (at chr 5 pos 874563 (W/874498-874563) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 16, R 15, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO375003.

SALKseq_044326.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G21400.1=UTR3 and AT2G21410.1=P355 (around chr 2 pos 9161664 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO415072.

SALKseq_044326.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G32950.1 and AT4G32960.1=UTR3 (at chr 4 pos 15907932 (W/15907868-15907932) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 16, R 15, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO368294.

SALKseq_044326.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G01800.2 (at chr 4 pos 776451 (W/776373-776451) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 16, R 15, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371648.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_044326

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662287 (SALK_044326C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G21400 AT2G21410 AT4G01800 AT4G32950 AT4G32960 At5g03495 AT5G03495 At5g03500

EMBL accession:

BH754917

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.