NASC

NASC ID: N544655

Name: SALK_044655

ABRC stock number: SALK_044655

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_044655.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G01150.1 (at chr 1 pos 71218 (C/71218-71272) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 17, R 14, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385059.

SALKseq_044655.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G61630.1 (at chr 3 pos 22805942 (W/22805899-22805942) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 39-67 bps onto the T-DNA. Pools P 17, R 14, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388405.

SALKseq_044655.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G05010.1 (at chr 4 pos 2567668 (W/2567617-2567668) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 17, R 14, C 20 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391738.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_044655

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664578 (SALK_044655C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G61630 AT4G05010 At1g01150

EMBL accession:

ED587550

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g01150

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.