NASC

NASC ID: N551265

Name: SALK_051265

ABRC stock number: SALK_051265

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: MGO3.3 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g60050.

Associated Polymorphisms:

SALKseq_051265.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G60050.1 (at chr 5 pos 24183855 (C/24183855-24183920) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 25, R 23, C 27 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO355246.

SALKseq_051265.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G44170.1=P355 and AT1G44180.1=UTR3 (at chr 1 pos 16801172 (W/16801095-16801172) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 25, R 23, C 27 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345211.

SALKseq_051265.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G68940.1 (at chr 1 pos 25924501 (W/25924468-25924501) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 42-68 bps onto the T-DNA. Pools P 25, R 23, C 27 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348555.

SALKseq_051265.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G25330.1=UTR3 and AT4G25340.1=UTR3 (at chr 4 pos 12958768 (C/12958768-12958846) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 25, R 23, C 27 N 30. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351912.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_051265

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662451 (SALK_051265C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G44170 AT1G44180 AT1G68940 AT4G25330 AT4G25340 At5g60050

EMBL accession:

ED589359

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.