NASC

NASC ID: N552866

Name: SALK_052866

ABRC stock number: SALK_052866

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: MUK11.16 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g04840.

Associated Polymorphisms:

SALKseq_052866.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G04840.1 (at chr 5 pos 1407272 (W/1407220-1407272) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 50-84 bps onto the T-DNA. Pools P 22, R 21, C 29 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO385449.

SALKseq_052866.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G35780.1 (at chr 1 pos 13277409 (W/13277359-13277409) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 49-84 bps onto the T-DNA. Pools P 22, R 21, C 29 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372050.

SALKseq_052866.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G41420.1 (at chr 2 pos 17267923 (W/17267859-17267923) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 68-95 bps onto the T-DNA. Pools P 22, R 21, C 29 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375405.

SALKseq_052866.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G17470.1 and AT2G17477=P355 (at chr 2 pos 7588676 (W/7588645-7588676) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 32-60 bps onto the T-DNA. Pools P 22, R 21, C 29 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO378752.

SALKseq_052866.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G09030.1=UTR3 and AT3G09032.1=UTR3 (at chr 3 pos 2756922 (W/2756872-2756922) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 22, R 21, C 29 N 30. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382096.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_052866

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662475 (SALK_052866C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G35780 AT2G17470 AT2G17477 AT2G41420 AT3G09030 AT3G09032 At5g04840

EMBL accession:

BH789950 ED589520

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.