NASC Stock Detail Page
NASC ID: N558434
Name: SALK_058434
ABRC stock number: SALK_058434
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. April 2002 TAIR gene annotation: T1E2.3 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g28050. Associated Polymorphisms: SALKseq_058434.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G28050.1 (at chr 2 pos 11939152 (W/11939097-11939152) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 34, R 32, C 34 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352277. SALKseq_058434.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G30040.1=UTR3 and AT2G30050.1=P355 (at chr 2 pos 12823212 (C/12823212-12823265) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 34, R 32, C 34 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355610. SALKseq_058434.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G30060.1 (at chr 2 pos 12826892 (W/12826836-12826892) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 34, R 32, C 34 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO358951. SALKseq_058434.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G11060.1 (at chr 4 pos 6754930 (C/6754930-6754984) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 34, R 32, C 34 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362296.
Donation Date: 2002-04-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_058434
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N659264 (SALK_058434C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.