NASC

NASC ID: N559180

Name: SALK_059180

ABRC stock number: SALK_059180

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F28O16.3 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g76660.

Associated Polymorphisms:

SALKseq_059180.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G76660.1 (at chr 1 pos 28769904 (W/28769869-28769904) on the TAIR10). The sequences' 1-36 bps mapped on the genome, 40-66 bps onto the T-DNA. Pools P 33, R 32, C 38 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375734.

SALKseq_059180.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G17010.1=UTR3 and AT1G17020.1=P355 (at chr 1 pos 5819476 (W/5819428-5819476) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 33, R 32, C 38 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379081.

SALKseq_059180.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G28365.1 (at chr 4 pos 14033928 (W/14033881-14033928) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 33, R 32, C 38 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382426.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_059180

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662617 (SALK_059180C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G17010 AT1G17020 AT4G28365 At1g76660

EMBL accession:

ED591016

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.