NASC

NASC ID: N559252

Name: SALK_059252

ABRC stock number: SALK_059252

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: K8K14.7 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g67350.

Associated Polymorphisms:

SALKseq_059252.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G67350.1 (at chr 5 pos 26869743 (W/26869682-26869743) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 33, R 40, C 32 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342260.

SALKseq_059252.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G59835.1 and AT1G59840.2=P355 (at chr 1 pos 22025987 (W/22025910-22025987) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 33, R 40, C 32 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412518.

SALKseq_059252.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G13950.1 (at chr 5 pos 4500961 (W/4500911-4500961) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 49-77 bps onto the T-DNA. Pools P 33, R 40, C 32 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345616.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_059252

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_059252C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G59835 AT1G59840 AT5G13950 At5g67350

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.