NASC

NASC ID: N559807

Name: SALK_059807

ABRC stock number: SALK_059807

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: F10D13.8 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g69410.

Associated Polymorphisms:

SALKseq_059807.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G69410.1 (at chr 1 pos 26089977 (W/26089914-26089977) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 34, R 34, C 33 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO348996.

SALKseq_059807.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G40310.1 and AT5G40316 (at chr 5 pos 16118914 (W/16118873-16118914) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 34, R 34, C 33 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352351.

SALKseq_059807.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G42635.1 and AT5G42640.1 (at chr 5 pos 17082852 (W/17082793-17082852) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 34, R 34, C 33 N 37. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355683.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_059807

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662636 (SALK_059807C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT5G40310 AT5G40316 AT5G42635 AT5G42640 At1g69410

EMBL accession:

BH791346

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.