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NASC Stock Detail Page

NASC ID: N560069

Name: SALK_060069

ABRC stock number: SALK_060069

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F17A9.9 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g06940.

Associated Polymorphisms:

SALKseq_060069.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G06940.1 (at chr 3 pos 2189825 (W/2189777-2189825) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 45-77 bps onto the T-DNA. Pools P 35, R 35, C 36 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359041.
SALKseq_060069.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G78120.1=P355 and AT1G78130.1=P355 (at chr 1 pos 29397978 (C/29397978-29398027) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 59-76 bps onto the T-DNA. Pools P 35, R 35, C 36 N 31. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO355701.

Donation Date: 2002-04-30

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_060069

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662643 (SALK_060069C).

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.