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NASC Stock Detail Page

NASC ID: N560387

Name: SALK_060387

ABRC stock number: SALK_060387

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_060387.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G49850.1 (at chr 3 pos 18489214 (W/18489162-18489214) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 34, R 39, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392538.

SALKseq_060387.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G57770.1=UTR3 and AT3G57780.1 (at chr 3 pos 21398969 (W/21398892-21398969) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 34, R 39, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO395889.

SALKseq_060387.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G24420.1=P355 and AT3G24430.1=UTR3 (at chr 3 pos 8865413 (W/8865336-8865413) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 34, R 39, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399230.

SALKseq_060387.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G36450.1 and AT4G36460.1 (at chr 4 pos 17212284 (W/17212208-17212284) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 34, R 39, C 32 N 32. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO402573.

Donation Date: 2002-04-30

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_060387

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662652 (SALK_060387C).

Status Price (£)
Available to order at NASC £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • TRB3
EMBL accession:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.