NASC

NASC ID: N560410

Name: SALK_060410

ABRC stock number: SALK_060410

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: T2H3.3 Exon At4g02230 T2H3.3 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At4g02230.

Associated Polymorphisms:

SALKseq_060410.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G02230.1 (at chr 4 pos 979695 (C/979695-979732) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 47-77 bps onto the T-DNA. Pools P 35, R 33, C 34 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO372460.

SALKseq_060410.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G51340.1 (at chr 1 pos 19034994 (W/19034952-19034994) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 43-72 bps onto the T-DNA. Pools P 35, R 33, C 34 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359062.

SALKseq_060410.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G19800.1 (at chr 1 pos 6846242 (W/6846165-6846242) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 35, R 33, C 34 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO362411.

SALKseq_060410.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G20620.1 (at chr 1 pos 7144003 (C/7144003-7144044) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 40-69 bps onto the T-DNA. Pools P 35, R 33, C 34 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO365760.

SALKseq_060410.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G02290.1 (at chr 4 pos 1005189 (W/1005130-1005189) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 35, R 33, C 34 N 35. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369105.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_060410

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662653 (SALK_060410C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G19800 AT1G20620 AT1G51340 AT4G02290 At4g02230

EMBL accession:

BH791533

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.