NASC

NASC ID: N561660

Name: SALK_061660

ABRC stock number: SALK_061660

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: F13P17.4 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g34200.

Associated Polymorphisms:

SALKseq_061660.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G34200.1 (at chr 2 pos 14441715 (W/14441666-14441715) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 36, R 35, C 39 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO345764.

SALKseq_061660.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G65540.1 (at chr 1 pos 24363058 (C/24363058-24363111) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 36, R 35, C 39 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412667.

SALKseq_061660.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G22800.1 (at chr 1 pos 8071794 (W/8071732-8071794) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 36, R 35, C 39 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO342409.

SALKseq_061660.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G21140.1 (at chr 3 pos 7412787 (C/7412787-7412859) on the TAIR10). The sequences' 1-73 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 36, R 35, C 39 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349108.

SALKseq_061660.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G08280.1=UTR3 and AT4G08290.1=P355 (at chr 4 pos 5233364 (W/5233333-5233364) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 31-58 bps onto the T-DNA. Pools P 36, R 35, C 39 N 39. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO352465.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_061660

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664643 (SALK_061660C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G22800 AT1G65540 AT3G21140 AT4G08280 AT4G08290 At2g34200

EMBL accession:

ED592071

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.