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NASC Stock Detail Page

NASC ID: N564462

Name: SALK_064462

ABRC stock number: SALK_064462

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_064462.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G33250.1 (at chr 1 pos 12059655 (W/12059606-12059655) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 47-78 bps onto the T-DNA. Pools P 39, R 40, C 38 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382744.

SALKseq_064462.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G59500.1 (at chr 1 pos 21856094 (C/21856094-21856171) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 39, R 40, C 38 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386095.

SALKseq_064462.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G64170.1 (at chr 1 pos 23818254 (W/23818192-23818254) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 39, R 40, C 38 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389443.

SALKseq_064462.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G71970.1 (at chr 1 pos 27093958 (C/27093958-27094019) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 39, R 40, C 38 N 40. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO392782.

Donation Date: 2002-05-31

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_064462

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662746 (SALK_064462C).

Status Price (£)
Available to order at NASC £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.