NASC Stock Detail Page
NASC ID: N569615
Name: SALK_069615
ABRC stock number: SALK_069615
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_069615.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G26000.1 (at chr 5 pos 9080395 (C/9080395-9080455) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 40, R 38, C 36 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383063. SALKseq_069615.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G54180.2=UTR3 and AT1G54200.1=P355 (at chr 1 pos 20231218 (C/20231218-20231270) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 51-78 bps onto the T-DNA. Pools P 40, R 38, C 36 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376370. SALKseq_069615.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G23620.1=P355 and AT2G23630.1=UTR3 (at chr 2 pos 10051825 (W/10051769-10051825) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 40, R 38, C 36 N 38. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379717.
Donation Date: 2002-05-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_069615
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_069615C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.