NASC

NASC ID: N571461

Name: SALK_071461

ABRC stock number: SALK_071461

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At2g35200. T4C15.13 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_071461.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G35200.1 (at chr 2 pos 14834198 (W/14834145-14834198) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 48, R 46, C 42 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389860.

SALKseq_071461.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G28960.1 and AT3G28970.1=UTR3 (at chr 3 pos 10987521 (C/10987521-10987586) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 48, R 46, C 42 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393213.

SALKseq_071461.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G60030.1 (at chr 3 pos 22169950 (W/22169898-22169950) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 48, R 46, C 42 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396551.

SALKseq_071461.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G16590.1=UTR3 and AT4G16600.1 (at chr 4 pos 9349941 (C/9349941-9349996) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 48, R 46, C 42 N 50. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399896.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071461

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N655605 (SALK_071461C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G28960 AT3G28970 AT3G60030 AT4G16590 AT4G16600 At2g35200

EMBL accession:

BH850559

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.