NASC

NASC ID: N571693

Name: SALK_071693

ABRC stock number: SALK_071693

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

June 2002 TAIR gene annotation - F10A5.13 Intron. At1g75680. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_071693.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G75680.1 (at chr 1 pos 28418395 (W/28418318-28418395) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 49, R 46, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356395.

SALKseq_071693.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G36790 and AT1G36795.1 (at chr 1 pos 13941115 (W/13941040-13941115) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 49, R 46, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO349716.

SALKseq_071693.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G36270.1 (at chr 2 pos 15207251 (W/15207191-15207251) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 55-76 bps onto the T-DNA. Pools P 49, R 46, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359743.

SALKseq_071693.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G38440 and AT1G38450.1=P355 (at chr 1 pos 14489796 (C/14489796-14489871) on the TAIR10). The sequences' 1-76 bps mapped on the genome. Pools P 49, R 46, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353071.

Donation Date: 2002-01-06

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071693

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662919 (SALK_071693C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G36790 AT1G36795 AT1G38440 AT1G38450 AT2G36270 At1g75680

EMBL accession:

BH850687

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.