NASC Stock Detail Page
NASC ID: N574256
Name: SALK_074256
ABRC stock number: SALK_074256
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. June 2002 TAIR gene annotation . Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_074256.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G23070.1 (at chr 5 pos 7742765 (C/7742765-7742823) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 56, R 54, C 60 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO389931.
SALKseq_074256.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G38290.1=UTR3 and AT4G38300.1 (at chr 4 pos 17944393 (C/17944393-17944443) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 46-79 bps onto the T-DNA. Pools P 56, R 54, C 60 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO386586.
Donation Date: 2002-01-06
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_074256
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_074256C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.