NASC

NASC ID: N575095

Name: SALK_075095

ABRC stock number: SALK_075095

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g02570 T14P4.2 3 Intron T14P4.2 3 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_075095.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G02575.1 (at chr 1 pos 543093 (C/543093-543136) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 60, R 51, C 60 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373208.

SALKseq_075095.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G22060.1 (at chr 1 pos 7781656 (W/7781578-7781656) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 60, R 51, C 60 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO376555.

SALKseq_075095.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G30945 (at chr 2 pos 13172123 (W/13172045-13172123) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 60, R 51, C 60 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO379903.

SALKseq_075095.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G37540.1=P355 and AT2G37550.1=UTR3 (at chr 2 pos 15754873 (W/15754845-15754873) on the TAIR10). The sequences' 1-29 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 60, R 51, C 60 N 56. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383249.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_075095

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666440 (SALK_075095C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G02575 AT1G22060 AT2G30945 AT2G37540 AT2G37550 At1g02570

EMBL accession:

BZ761697

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.