NASC Stock Detail Page
NASC ID: N578406
Name: SALK_078406
ABRC stock number: SALK_078406
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At5g61260. MFB13.3 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_078406.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G61250.2=P355 and AT5G61260.1 (at chr 5 pos 24636954 (C/24636954-24637001) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430230. SALKseq_078406.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G11572.1=UTR3 and AT1G11580.1=P355 (at chr 1 pos 3887747 (W/3887685-3887747) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423598. SALKseq_078406.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G01230.1 (at chr 3 pos 77589 (W/77553-77589) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426914. SALKseq_078406.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G22420.1 and AT5G22430.1=P355 (at chr 5 pos 7433354 (W/7433293-7433354) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433536.
Donation Date: 2002-01-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_078406
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666490 (SALK_078406C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.