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NASC Stock Detail Page

NASC ID: N578406

Name: SALK_078406

ABRC stock number: SALK_078406

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At5g61260. MFB13.3 L300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_078406.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G61250.2=P355 and AT5G61260.1 (at chr 5 pos 24636954 (C/24636954-24637001) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430230.

SALKseq_078406.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G11572.1=UTR3 and AT1G11580.1=P355 (at chr 1 pos 3887747 (W/3887685-3887747) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423598.

SALKseq_078406.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G01230.1 (at chr 3 pos 77589 (W/77553-77589) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426914.

SALKseq_078406.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G22420.1 and AT5G22430.1=P355 (at chr 5 pos 7433354 (W/7433293-7433354) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 65, R 66, C 66 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433536.

Donation Date: 2002-01-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_078406

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666490 (SALK_078406C).

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.