NASC

NASC ID: N580011

Name: SALK_080011

ABRC stock number: SALK_080011

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2003 TAIR gene annotation -At1g28080 F13K9.18 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_080011.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G28080.1 (at chr 1 pos 9790354 (W/9790322-9790354) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 68, R 63, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433769.

SALKseq_080011.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G26610.1 (at chr 2 pos 11323218 (W/11323170-11323218) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 68, R 63, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO437073.

SALKseq_080011.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G46730.1 (at chr 3 pos 17213525 (W/17213473-17213525) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 68, R 63, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417201.

SALKseq_080011.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G19530.1=P355 and AT3G19540.1=P355 (at chr 3 pos 6777956 (W/6777879-6777956) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 68, R 63, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420517.

SALKseq_080011.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G30860.1 (at chr 4 pos 15026695 (C/15026695-15026772) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 68, R 63, C 63 N 67. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423833.

Donation Date: 2003-04-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_080011

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_080011C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G26610 AT3G19530 AT3G19540 AT3G46730 AT4G30860 At1g28080

EMBL accession:

CC054736

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.