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NASC Stock Detail Page

NASC ID: N580084

Name: SALK_080084

ABRC stock number: SALK_080084

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2003 TAIR gene annotation -At5g08580 MAH20.14 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_080084.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G08590.1 (at chr 5 pos 2783182 (C/2783182-2783231) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 41-66 bps onto the T-DNA. Pools P 69, R 63, C 68 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430478.
SALKseq_080084.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G55250.4=UTR3 and AT1G55260.2=P355 (at chr 1 pos 20613010 (W/20612957-20613010) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 49-79 bps onto the T-DNA. Pools P 69, R 63, C 68 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427162.

Donation Date: 2003-04-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_080084

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N657974 (SALK_080084C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.