NASC Stock Detail Page
NASC ID: N582090
Name: SALK_082090
ABRC stock number: SALK_082090
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. February 2003 TAIR gene annotation - At1g14360 F14L17. 13 R300-5' F14L17. 13 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_082090.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G14360.1 (at chr 1 pos 4913094 (W/4913052-4913094) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 45-71 bps onto the T-DNA. Pools P 72, R 74, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427534. SALKseq_082090.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G33860.1=P355 and AT2G33870.1 (at chr 2 pos 14330531 (W/14330488-14330531) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 39-74 bps onto the T-DNA. Pools P 72, R 74, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430851. SALKseq_082090.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G61070.1 (at chr 5 pos 24572817 (W/24572784-24572817) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 72, R 74, C 74 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434156.
Donation Date: 2003-03-11
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_082090
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666545 (SALK_082090C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.