NASC

NASC ID: N582269

Name: SALK_082269

ABRC stock number: SALK_082269

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_082269.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G48095.1 (at chr 1 pos 17761433 (W/17761374-17761433) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 71, R 79, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424254.

SALKseq_082269.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G56418.1 (at chr 1 pos 21122070 (W/21122014-21122070) on the TAIR10). The sequences' 1-57 bps mapped on the genome. Pools P 71, R 79, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427569.

SALKseq_082269.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G19200.1=P355 and AT3G19210.1=UTR3 (at chr 3 pos 6652447 (C/6652447-6652511) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 71, R 79, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430886.

SALKseq_082269.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G25710.1=UTR3 and AT3G25715 (at chr 3 pos 9372148 (W/9372112-9372148) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 71, R 79, C 77 N 76. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434191.

Donation Date: 2002-11-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_082269

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_082269C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G48095 AT1G56418 AT3G19200 AT3G19210 AT3G25710 AT3G25715 At2g39500

EMBL accession:

BZ761838

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.