NASC Stock Detail Page
NASC ID: N583322
Name: SALK_083322
ABRC stock number: SALK_083322
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At1g60040. T2K10.9 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_083322.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G60040.1 and AT1G60050.1 (at chr 1 pos 22120116 (W/22120056-22120116) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 73, R 77, C 71 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424446. SALKseq_083322.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28350.1=UTR3 and AT2G28355.1=P355 (at chr 2 pos 12117253 (W/12117176-12117253) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 73, R 77, C 71 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427761. SALKseq_083322.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28650.1=P355 and AT2G28660.1=P355 (at chr 2 pos 12292952 (C/12292952-12292985) on the TAIR10). The sequences' 1-34 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 73, R 77, C 71 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431078. SALKseq_083322.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G20460.1=UTR3 and AT3G20470.1=UTR3 (at chr 3 pos 7140095 (C/7140095-7140146) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 73, R 77, C 71 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434383.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_083322
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666564 (SALK_083322C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.